For this study, mature cerebral organoids (6-7 months old) were transferred to a Mesh MEA using a polyethyleneimine and laminin coating protocol and BrainPhys cell culture media. After 3 weeks in culture, COs displayed higher electrical activity on the Mesh MEA compared to planar MEA chips, which contained either flat or 3D-spike electrodes.
In the Abilez et al. study, the HEKA EPC 10 Patch Clamp Amplifier provided the sensitivity, stability, and control needed to investigate individual cells within the complex 3D environment of the organoid.
Retinitis pigmentosa (RP) and retinal dystrophy are debilitating genetic disorders that lead to progressive vision loss due to the degeneration of photoreceptor cells in the retina. Despite advances in genetic research, effective treatments remain limited, making the development of innovative research models crucial. Organoids and Mesh MEA (microelectrode array, also referred to as multielectrode array) technology from Multi Channel Systems have emerged as powerful tools in studying these diseases, providing insights into retinal function and potential therapeutic approaches.
The integration of behavioral telemetry and organoid research is transforming neuroscience and biomedical science by linking cellular models with complex biological behaviors.
Unlike traditional MEA recordings that can only measure field potentials, the IntraCell uses a laser to precisely optoporate cardiomyocytes underneath each electrode.
