Biochrom Bio 31+ Protein Hydrolysate Amino Acid Analyzer Systems
Biochrom Bio 31+ Protein Hydrolysate Amino Acid Analyzer Systems
The Bio 31+ Amino Acid Analyzer complies with the global pharmacopoeia guidelines for dedicated amino acid analysis by ion exchange liquid chromatography and post-column derivatization of ninhydrin positive substances. Used in various application areas, the Biochrom amino acid analyzer system plays a critical role in the ICH Q6B guidelines for amino acid characterization of biopharmaceutical ingredients.
Why you need a dedicated instrument for amino acid analysis:
Conventional HPLC systems face challenges in amino acid analysis due to sample complexity and unique chemical properties. A dedicated AAA system provides a reliable, consistent, and reproducible solution without the need for method development. Its pre-set and extensively tested configuration allows 24/7 routine use with minimal user intervention. Efficient screening of numerous samples for specific amino acids can be swiftly conducted around the clock using concise methods.
System suitable for:
- Research, development and manufacturing
- Quality control and compositional analysis
Specific applications:
- Detection of up to 20 amino acids normally found in proteins
- Protein and peptide analysis for pharmaceutical applications
- Food, beverages, plant extracts, fermentation products, culture media, nutraceuticals, and infusion fluids
Biochrom 31+ Series of Amino Acid Analyzers are customizable.
Please contact your sales representative or distributor for system ordering information.
The Bio 31+ Protein Hydrolysate System, utilizing sodium citrate-based buffers, ensures fully automated, high-standard analysis for generic drugs and infusion fluids. Our dedicated amino acid analyzers accurately quantify amino acids in solutions derived from diverse materials, including proteins, peptides, and pharmaceutical ingredients. This typically involves simple sample preparation methods like acid hydrolysis, eliminating the need for chemical derivatization before injecting the hydrolyzed sample, thanks to automated post-column ninhydrin detection.
The long-lasting sodium columns in the Bio 31+ Protein Hydrolysate System tolerate various matrices and high-salt content, making it effective for analyzing infusion liquids, recombinant peptides, and synthetic drugs. Its capability to handle complex sample matrices makes it as a robust quality control technique for analyzing vitamins, synthetic drugs, and more.
A Dedicated Amino Acid Analysis System
| Accurate: | Superb separation, accurately identifies and quantifies free amino acids, as well as the amino acid composition of proteins and peptides. |
| Reliable: | Minimal matrix interference and near baseline separation. Automated derivatization of amino acids maximizes reproducibility. |
| Fast: | Rapid protocols that increase sample throughput by cutting analysis time. |
| Economical: | The column cleaning and repacking service, gives columns an almost indefinite life and cuts the cost of ownership. |
| Fully Automated: | Total PC control allows 24/7 operations. |
| Versatile: | Wide variety of sample types compatible: Protein hydrolysates from various food and feedstuff samples or synthetic drugs, nutraceuticals, beverages. |
| Easy to implement: | No lengthy method development required as this well characterized system is established worldwide. All customers have their instrument optimized to their application directly from the factory. |
| Convenient: | Interchangeable sample trays allow samples to be prepared in advance and stored refrigerated. |
| Robust: | Advanced fluidics and robust hardware mean easy maintenance for users, backed by the reassurance of Biochrom's dedicated service and support team. |
| Flexible: | Integrated software, choice of different methods to adapt to the user's needs. Automatic sample set-up. Networking and exporting of data. |
| Compact: | Bench top design requiring minimum lab space. |
Instrument Typical Package
The Instrument package consists of the Analyzer (electronics unit, pumps, and detection system) plus:
- Cooled or Non-Cooled Autosampler
- Column kit: analytical column and top-up ion exchange resin, prewash column and top-up ion exchange resin
- Ninhydrin
- Buffer Chemical Kit suitable for up to 170 analyses
- Spare parts and Consumables Kit
- Calibration Standard
- Hardware control and data handling software
- Desktop PC, LED widescreen monitor
- User Manual (PDF format)
- Power cables
For buffer and chemicals information, consult the Consumables tab below.
| Instrument Specifications | ||
| Specification | Data | |
| Reproducibility | Area: <1.5% RSD at 5 nanomoles Retention time: < 0.5% RSD |
|
| Pumps | Two dual piston pumps with integrated pressure transducer and drain valve Ceramic pump head, sapphire pistons and valves Electronically controlled piston wash |
|
| Operating Pressures | Buffer pressure: maximum 150 bar Ninhydrin pressure: maximum 24 bar |
|
| Analytical Column | High pressure PEEK column packed with Ultropac 8 cation exchange resin. Peltier heating/cooling system |
|
| Sample Injection | 3 injection modes (full loop, partial loop and micro), 84 position autosampler Sample volumes from 1 µL to 5000 µL (200 µL loop supplied as standard) |
|
| Temperatures | Column temperature variable between 20°C and 99°C. Reaction coil temperature adjustable between 40°C and 145°C (135°C nominal for UltraNynhydrin, 138°C nominal for EzNin) |
|
| Software | Phoenix Integrated Control Software Data Apex Clarity or Agilent OpenLAB CDS EZChrom Edition |
|
| Photometer | Single flow cell with optical beam splitter with detection at 440 nm and 570 nm | |
| Weight and Dimensions | Main System: 50 kg (110 lb.) 48 x 59 x 57 cm (19 x 23 x 22 in.) Autosampler: 21kg (46 lb.) 30 x 57.5 x 36 cm (12 x 23 x 14 in.) |
|
| Required Services | Oxygen free nitrogen gas (99.99%) or argon gas regulated to 5 bar Drainage facility 240V/100V, 50Hz/60Hz, 300VA mains supply |
|
| Safety Systems |
Automatic shut down and reaction coil flushing in the event of:
|
|
| Related Documents | ||
| Biochrom Phoenix Brochure | DOWNLOAD | |
| Material Safety Data Sheet (MSDS) | View MSDS | |
| Certificates of Analysis | View Certificates of Analysis | |
Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 2L bottle, In-line filter, Quick Start Guide
Sufficient for up to 180 runs (Protein Hydrolysate System) or 90 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 2x 2L bottle, 2x In-line filter, Quick Start Guide
Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System)
Includes: EZ Nin Reagent ready-to-use 4x 2L bottle, 4x In-line filter, Quick Start Guide
Sufficient for up to 90 runs (Protein Hydrolysate System) or 45 runs (Physiological Fluid High Performance System)
Includes Ultra Ninhydrin Solution 2L, Ultrosolve Plus Solution, In-line Filter
Sufficient for up to 360 runs (Protein Hydrolysate System) or 180 runs (Physiological Fluid High Performance System)
Includes 4x Ultra Ninhydrin Solution 2L, 4x Ultrosolve Plus Solution, 4x In-line Filter
Sufficient for up to 320 runs (High Performance System)
Includes 2x Sodium Buffer 1, 2x Sodium Buffer 2, 2x Sodium Buffer 5, 1x Sodium Regeneration Buffer 6, 1x Sodium Loading Buffer
Sufficient for up to 350 runs
Includes 2x Accelerated Buffer 1, 1x Sodium Accelerated Buffer 2, 2x Sodium Accelerated Buffer 3, 2x Sodium Accelerated Buffer 4, 1x Sodium Loading Buffer
2 L, pH 3.2
2 L, pH 4.25
2 L, pH 6.45
1 L, NaoH
2 L, Ph 2.20
2 L
2 L
2 L
2 L
This ISO 17034 Reference Material (RM) is manufactured and verified in accordance system ISO17034 and ISO17025 requirements. The analyte concentrations are verified by a quality control laboratory and the true value and uncertainty values at the 95% confidence level are reported for each analyte.
This is supplied in a sealed glass vial containing 5 mL of standard solution in a 0.1 N Hydrochloric Acid matrix. A Certificate of Analysis is provided with each vial. Store Refrigerated (2o to 8oC) Do Not Freeze.
20cm x 4.6mm ID
20cm x 4.6mm ID
10cm x 4.6mm ID
All analytical columns
All prewash columns